Mammalian Cell Culture and Analysis
UCL East - Marshgate
14 - 16 January 2025
£1,950
This hands-on course teaches laboratory scientists and engineers how to handle and maintain mammalian cells applying appropriate aseptic techniques as well as how to evaluate cell quality using a range of analytical assays.
It provides a practical understanding of how to recover, cultivate, and preserve mammalian cells applying aseptic techniques at conventional laboratory scale. Training is focused on the hands-on application of industry relevant Standard Operating Procedures (SOPs) which are enhanced by relevant virtual reality simulations. Tutorials for SOPs, data analysis, and troubleshooting, will be supported by a series of introductory and expert lectures that will enable you to:
Apply basic aseptic techniques relevant to the culture and passaging of mammalian cells.
Perform procedures for preserving mammalian cells and retrieving those from frozen (liquid nitrogen) storage for subsequent cultivation.
Quantify cell numbers using a manual haemocytometer and an automated cell counter.
Assess the viability of mammalian cells using conventional Trypan blue exclusion assay and fluorescent dyes for labelling live and dead cells.
Identify the typical morphologies of mammalian cells and examine cell activity by immunocytochemistry and image analysis software.
Recognise conventional cell culture equipment including Carbon Dioxide incubators and Class II Biological Safety Cabinets (BSCs) and apply that equipment for the culture of mammalian cells.
Apply tissue culture and analytical laboratory safety procedures and recognise the importance of those protocols to safeguard laboratory personnel.
Apply biological waste management protocols appropriate for all aspects of mammalian cell culture and analysis.
This course is recommended for:
New entrants to the biopharmaceutical sector or those transitioning from other roles who need to gain practical, hands-on mammalian cell culture skills including: research and development scientists; bioprocess and manufacturing engineers; bioprocess equipment application specialists; and laboratory and quality control technicians.