Mastering Protein Purification Using Chromatography Techniques
FLEXBIO, Heriot-Watt University, Edinburgh
11 - 13 February 2025
(FREE)
This 
course is aimed at anyone who wants to develop an understanding of theory and techniques in protein purification using fast protein liquid chromatography (FPLC) on an ÄKTA chromatography system. The course, while specifically designed around the ÄKTA Avant system, provides comprehensive insights and practical knowledge that will be beneficial to all ÄKTA users, regardless of the specific model they are using. This training course will also focus on ÄKTA maintenance and cleaning and the applications of using FPLC.
The 3-day course consists of lectures, practical demos and hands-on exercises, hosted by the Industrial Biotechnology Innovation Centre (IBioIC). On completion of the course, the attendees will be presented with a certificate of course completion by IBioIC.
Training will be conducted in our Centre of Excellence facility housing cutting-edge laboratory instruments and benchtop-scale processing equipment, allowing delegates to gain vital hands-on practical exposure to industry-standard biomanufacturing processes and procedures.
Learning Outcomes:
Learn the basic principles and applications of different chromatography techniques, such as affinity, ion exchange, hydrophobic interaction and size exclusion
Learn how an ӒKTA chromatography system works, how to prepare for a chromatography run, and importantly, how to ensure your ӒKTA chromatography system remains clean
Purify green fluorescent protein (GFP) using hydrophobic interaction chromatography (HIC) and Ion exchange chromatography (IEX). Qualitative and quantitative analysis of purified protein using SDS-PAGE and UV-Vis spectroscopy
Learn how to pack chromatography columns like a pro through a live demonstration and discover why it matters for your chromatography results
Who should attend:
Scientists, manufacturing operators, engineers and managers wishing to gain knowledge of conventional upstream bioprocessing operations and their practical application in industry.